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Vol. 32. Núm. 3.
Páginas 115-120 (Marzo 2008)
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Vol. 32. Núm. 3.
Páginas 115-120 (Marzo 2008)
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DOI: 10.1016/S0210-5691(08)70921-5
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Comparison of bronchoscopic bronchoalveolar lavage vs blind lavage with a modified nasogastric tube in the etiologic diagnosis of ventilator-associated pneumonia
Comparación de lavado broncoalveolar broncoscópico frente a lavado ciego con sonda nasogástrica modificada en el diagnóstico etiológico de neumonía asociada a ventilador
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A. Leoa, J. Galindo-galindoa, E. Folchb, A. Guerreroa, F. Bosquesa, R. Mercadoa, A.C. Arroligac,
Autor para correspondencia
aarroliga@swmail.sw.org

Correspondence: Dr. A.C. Arroliga. Scott & White Hospital. 2401 South 31st Street. Temple, TX 76508. USA.
a Hospital Universitario Dr. José Eleuterio González. Monterrey. México. USA
b Department of Pulmonary, Allergy and Critical Care Medicine. The Cleveland Clinic. Cleveland. Ohio. USA
c Division of Pulmonary and Critical Care Medicine. Scott & White Hospital. Temple. Texas. USA
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Tablas (4)
Table 1. Clinical characteristics of study subjects
Table 2. Comparison of culture results for bronchoscopic and non-bronchoscopic procedures in patients with significant growth (> 104cfu)
Table 3. Comparison of culture results for bronchoscopic and non-bronchoscopic procedures in patients with non-significant growth (< 104cfu)
Table 4. Performance characteristics of non-bronchoscopic technique against the standard of bronchoscopic technique
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Objective

Our objective was to compare the results of a blind lavage vs a bronchoscopic-guided bronchoalveolar lavage for the etiologic diagnosis of ventilator-associated pneumonia (VAP).

Design

Prospective study in consecutive patients with high probability of VAP. Every patient underwent both procedures, in a formally randomized fashion. The interpretation of quantitative cultures was done in a blind fashion.

Setting

Single center study, with a 20 bed medical and surgical Intensive Care Unit of the University Hospital in Monterrey, Mexico.

Patients

Twenty-five patients with high probability of VAP.

Interventions

Every patient underwent blind bronchoalveolar lavage with a modified nasogastric tube, and a bronchospic-guided bronchoalveolar lavage.

Results

Twenty-one patients underwent both procedures. Four patients were excluded due to contamination of the cultures. The quantitative cultures were compared in a paired fashion. Only two patients had discordant cultures. The correlation coefficient between the number of colonies was very high, r=0.90 (95% confidence interval [CI], 0.77-0.96; p=0.0001).

Conclusions

The blind bronchoalveolar lavage with a modified nasogastric tube is a valuable tool for the identification of etiologic agent in VAP, particularly when trained bronchoscopists or the necessary resources for bronchoscopic-guided bronchoalveolar lavage are not readily available.

Key words:
ventilator-associated pneumonia
bronchoalveolar lavage
nasogastric tube
blind bronchoalveolar lavage
Objetivo

Nuestro objetivo fue el de comparar los resultados de un lavado ciego frente a un lavado broncoalveolar guiado con broncoscopio para el diagnóstico etiológico de neumonía asociada a ventilador (NAV).

Diseño

Estudio prospectivo en pacientes consecutivos con alta probabilidad de NAV. En todos los pacientes se llevaron a cabo ambos procedimientos de manera aleatorizada. La interpretación de los cultivos cuantitativos fue hecha a ciegas.

Ámbito

Estudio en un único centro, en una Unidad de Cuidados Intensivos Quirúrgicos con 20 camas del Hospital Universitario de Monterrey, en México.

Pacientes

Veinticinco pacientes con alta probabilidad de NAV.

Intervenciones

A cada paciente se le realizó un lavado broncoalveolar ciego con una sonda naguiado con broncoscopio.

Resultados

Se realizaron ambos procedimientos en 21 pacientes. Cuatro fueron excluidos debido a contaminación de los cultivos. Los cultivos cuantitativos fueron comparados en pares. Solo dos pacientes tenían cultivos discordantes. El coeficiente de correlación entre el número de colonias fue muy alto, r=0,90 (intervalo de confianza [IC] del 95% 0,77-0,96; p=0,0001).

Conclusiones

El lavado broncoalveolar ciego con sonda nasogástrica modificada es una herramienta de mucho valor para la identificación del agente etiológico en NAV, especialmente cuando un broncoscopista experto o los recursos necesarios para lavado broncoalveolar guiado con broncoscopio no están fácilmente disponibles.

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INTRODUCTION

Ventilator-associated pneumonía (VAP) is a major medical problem. VAP is associated with longer stay in the Intensive Care Unit (ICU) and in the hospital, high cost, and high mortality rates1–5. The clinical diagnosis of VAP that includes radiographic changes and at least one clinical finding such as fever, leukocytosis, or purulent tracheal aspirate has a good sensitivity but poor specificity4. It has been recommended that a sample of the lower respiratory tract should be obtained when the clinical diagnoses of VAP is suspected4. Invasive diagnostic testing may increase the confidence of the clinician in the diagnosis and management of the VAP and because of the good negative predictive value thus allowing discontinuation of antibiotic therapy4,6.

Quantitative cultures of specimens taken with bronchoscopic techniques or through blind non-bronchoscopic techniques are appealing alternatives for the collection of lower respiratory tract specimens4. A strategy of diagnosis that includes obtaining a sample by bronchoscopic technique is associated with a lower mortality at day 14 and a decrease in antibiotic use7. However, bronchoscopy may not be readily available4, especially in countries with limited resources, therefore diagnostic testing with non-bronchoscopic techniques and early use of broad-spectrum antibiotics are attractive options in order to minimize cost, antibiotic use, as well as improve survival8.

The hypothesis of the study is that the isolated bacteria and quantitative cultures would be similar in the fluid obtained with both techniques. The goal of this study was to compare the microbiologic findings in lower airway lavage through a non-bronchoscopic technique using a modified nasogastric tube9 against a fiberoptic bronchoscope-directed bronchoalveolar lavage (BAL) in the same patient.

PATIENTS AND METHODS

We prospectively studied 25 patients with suspected VAP in the Medical and Surgical ICU at Hospital Universitario Dr. José Eleuterio González between June 1, 2005 and June 1, 2006. This hospital is a major teaching hospital in Monterrey, Mexico. The unit has 20 beds, staffed by residents in Internal Medicine, Fellows in Pulmonary and Critical Care, and Attending Intensive Care specialists. The ratio of patients per nurses is 2:1.

Patients were eligible for the study if they met the following inclusion criteria: ≥ 18 year-old, intubated for more than 48 hours, who met the clinical definition for VAP. Clinical definition of VAP refers to new infiltrates in the chest X-ray with ≥ 2 of the following: fever or hypothermia (≤ 35° C or ≥ 38.3° C), leukocytosis or leukopenia (≥ 12,000/dl or ≤ 4,000/dl), purulent secretions in the endotracheal tube (ETT), and/or poor oxygenation (PaO2/FiO2 ≤ 240mmHg)1,4. Patients were excluded if they had a contraindication for bronchoscopy (for example severe hypoxemia PaO2/FiO2 < 100mmHg), refractory coagulopathy (prothrombin time and partial thromboplastin time > twice the upper limit of normal nonresponsive to 10cc/kg of fresh frozen plasma [FFP]), or hemodinamically unstable at time of bronchoscopy (mean arterial pressure [MAP] < 60mmHg).

The study was approved by the Institutional Review Board, and informed consent was obtained from the patient or next of kin.

The following characteristics were recorded prospectively at the time of ICU admission: age, sex, APACHE II score10, main diagnosis and comorbidities, clinical pulmonary infection score (CPIS)11, previous antibiotics, concurrent extrapulmonary infections, and radiologic findings. The CPIS and APACHE II scores were calculated again on the day of the study. Every study participant underwent two procedures:

  • 1.

    Blind BAL with a modified nasogastric tube (non-bronchoscopic).

  • 2.

    BAL using a standard fiberoptic bronchoscope.

The modification in the nasogastric tube consists in the cutting of the tip of the catheter to remove the area with multiple holes9. We randomly determined which procedure was done first. The procedures were performed 20 minutes apart for stabilization purposes.

Patients were sedated and preoxygenated while on continuous pulse oxymetry monitoring. For the nonbronchoscopic procedure, a 14F nasogastric tube was slowly introduced through an adaptor (Portex®, Keene, New Hampshire, USA) into the ETT, until resistance was felt. Three aliquots of 50ml of sterile 0.9% saline were instilled sequentially, and withdrawn by manual suction with a 50ml catheter-tip piston syringe. The first aspirate was discarded. The remaining two aspirates were processed in the Microbiology laboratory. In the bronchoscopic procedure, after tracheal aspiration, the fiberoptic bronchoscope (Pentax®, Orangeburg, New York, USA) was introduced into the ETT via the ETT adaptor and positioned («wedged») in the orifice of the sampling area with sequential instillation of three aliquots of 50ml of sterile 0.9% saline. The first aspirate was discarded, and remaining fluid was sent to the Microbiology laboratory. No aspiration was done through the working channel of the bronchoscope before the collection of samples in order to minimize contamination.

The specimens were immediately sent to the laboratory and processed according to previously described methods by Baselski12. The samples were centrifuged for 30 seconds, a Gram stain was done searching for intracellular organisms. The bacterial cultures were processed with microorganisms quantified by an experienced microbiologist using standard serial dilution and the results were expressed as colony-forming units (cfu/ml)13. The cut-off point for significant growth was 104cfu/ml for both procedures14,15. The microbiologist reading the cultures was blinded for which procedure was used and for the result of the corresponding sample.

Statistical analysis

Descriptive statistics were used, with all comparisons being paired, and all tests of significance two tailed. All values are expressed as the mean ± standard deviation (SD). Sensitivity, specificity, positive predictive value, negative predictive value, and likelihood ratios were determined considering fiberoptic bronchoscopy (FOB) with BAL as the gold standard. Correlation coefficients were also calculated.

RESULTS

Twenty-five eligible patients were included in the study, with 4 patients being excluded from analysis due to contamination of microbiology cultures. The 21 subjects (14 men and 7 women) had a mean age of 42 years-old (range 17–82), with mean APACHE II score of 15 (± 8) and a CPIS of 7.5 on the day of the procedures (table 1). The reasons for hospital admission were pneumonia (5), trauma (4), sepsis (2), stroke (1), burns (1), severe pancreatitis (1), eclampsia (1), brain tumor (1), empyema (1), aortic aneurysm (1), pulmonary hemorrhage (1), hypovolemic shock (1), and respiratory failure (1). All patients were intubated, and 15 of 21 had been receiving antibiotics for the reason that prompted their admission to the ICU.

Table 1.

Clinical characteristics of study subjects

Characteristic  Mean ± SD 
Age, year (range)  42 (17–82) 
Sex (male)  14 
APACHE II on admission  13 ± 7 
APACHE II at time of procedure  15 ± 8 
PaO2/FiO2  213 ± 81 
CPIS on the day of study  7.5 ± 1.54 
Days intubated  8 ± 4 
Temperature (º C)  38.1 ± 0.81 
White blood cell count (×109/l)  16 ± 9.1 
Radiologic pattern
Diffuse  14 (66%) 
Focal  7 (33%) 

CPIS: Clinical Pulmonary Infection Score; SD: standard deviation.

Every patient underwent both bronchoscopic and non-bronchoscopic procedures. The mean volume of fluid recovered from lavage was 42 ± 8ml in the bronchoscopic technique and 40 ± 10ml in the nonbronchoscopic technique. With the bronchoscopic technique, significant growth was found in 66.7% (n = 14) of the samples and non-significant growth in 33.3% (n = 7). With the non-bronchoscopic technique significant growth was found in 71.4% (n = 15) of the samples, non-significant growth in 28.6% (n = 6) (tables 2 and 3).

Table 2.

Comparison of culture results for bronchoscopic and non-bronchoscopic procedures in patients with significant growth (> 104cfu)

Patient  Diagnosis  Non-bronchoscopic (blind)  Bronchoscopic 
Pneumonia  Pseudomonas aeruginosa  Pseudomonas aeruginosa 
3Brainstem tumor  Acinetobacter baumannii  Acinetobacter baumannii 
  Escherichia coli  Escherichia coli 
  Staphylococcus aureus  Staphylococcus aureus 
4Pneumonia  Staphylococcus aureus  Staphylococcus aureus 
  Klebsiella pneumoniae  Klebsiella pneumoniae 
6Severe pancreatitis  Pseudomonas aeruginosa  Pseudomonas aeruginosa* 
  Staphylococcus aureus  Staphylococcus aureus 
7Burns and pneumonitis  Pseudomonas aeruginosa  Pseudomonas aeruginosa 
  Staphylococcus aureus  Staphylococcus aureus 
  Stenotrophomonas maltophilia  Stenotrophomonas maltophilia 
8Abdominal aortic aneurysm  Acinetobacter baumannii  Acinetobacter baumannii 
  Staphylococcus sciuri  Staphylococcus sciuri 
9Pneumonia  Acinetobacter baumannii  Acinetobacter baumannii 
  Staphylococcus aureus  Staphylococcus aureus 
10Pulmonary hemorrhage  Acinetobacter baumannii  Acinetobacter baumannii 
  Citrobacter freundii  Citrobacter freundii 
11Hypovolemic shock  Acinetobacter baumannii  Acinetobacter baumannii 
  Staphylococcus aureus  Staphylococcus aureus 
12Sepsis  Acinetobacter baumannii  Acinetobacter baumannii 
  Staphylococcus aureus  Staphylococcus aureus 
14Pneumonia  Pseudomonas aeruginosa  Pseudomonas aeruginosa 
  Staphylococcus aureus  Staphylococcus aureus 
15Sepsis  Acinetobacter baumannii  Acinetobacter baumannii 
  Stenotrophomonas maltophilia  Stenotrophomonas maltophilia 
  Pseudomonas aeruginosa  Pseudomonas aeruginosa 
16Trauma  Staphylococcus aureus  Staphylococcus aureus 
  Stenotrophomonas maltophilia  Stenotrophomonas maltophilia 
  Acinetobacter baumannii  Acinetobacter baumannii 
19Trauma  Staphylococcus aureus  Staphylococcus aureus 
  Pseudomonas aeruginosa   
20Trauma  Staphylococcus aureus  Staphylococcus aureus 
    Escherichia coli 
*

The bronchoscopic technique showed growth below the threshold (< 104cfu) and was considered negative. cfu: colony-forming units.

Table 3.

Comparison of culture results for bronchoscopic and non-bronchoscopic procedures in patients with non-significant growth (< 104cfu)

Patient  Diagnosis  Non-bronchoscopic (blind)  Bronchoscopic 
HAP  Acinetobacter baumannii  Acinetobacter baumannii 
5Eclampsia  Pseudomonas putida  Pseudomonas putida 
  Enterobacter cloacae  Enterobacter cloacae 
13  Respiratory acidosis  Normal flora  Normal flora 
17  Trauma  No growth  No growth 
18Empyema  Klebsiella pneumoniae  Klebsiella pneumoniae 
  Acinetobacter baumannii  Acinetobacter baumannii 
  Staphylococcus aureus  Staphylococcus aureus 
21  Stroke  Enterobacter aerogenes  Enterobacter aerogenes 

cfu: colony-forming units; HAP: hospital-acquired pneumonia.

The quantitative cultures obtained through either technique are shown in table 3. Only two patients had discordant cultures, the non-bronchoscopic technique failed to provide quantitative evidence of infection in one case, and it identified a second organism inanother patient, that was not isolated with the bronchoscopic technique.

The most common isolated organisms were Staphylococcus aureus, Acinetobacter baumannii, and Pseudomonas aeruginosa with polymicrobial infection present in 16 (76.2%) cases, and single organism infection present in 14.3% of the cases. The Spearman's coefficient of rank correlation (r) for number of colonies showed a positive correlation at 0.90 (confidence interval [CI] 0.77-0.96; p = 0.0001) between the two techniques. The sensitivity of the non-bronchoscopic technique was 93%, and the specificity was 85% when compared to the bronchoscopic-guided bronchoalveolar lavage (table 4). We calculated the likelihood ratios16 or how many times more likely patients with the disease are to have that particular result than patients without the disease. The positive and negative likelihood ratios showed strong evidence to rule in or out the presence of VAP in this group of patients.The procedures were tolerated well with no episodes of desaturation below 88% with either technique.

Table 4.

Performance characteristics of non-bronchoscopic technique against the standard of bronchoscopic technique

  Non-bronchoscopic technique (95% CI) 
Sensitivity  92.8% (64-99%) 
Specificity  85% (42-99%) 
Positive predictive value  92% (64-99%) 
Negative predictive value  85% (42-99%) 
Positive likelihood ratio (conventional)  6.5 (1–40) 
Positive likelihood ratio (weighted)  13 (7.5-22.3) 
Negative likelihood ratio (conventional)  0.08 (0.012-0.57) 
Negative likelihood ratio (weighted)  0.16 (0.025-1.07) 

CI: confidence interval.

DISCUSSION

In this single center, prospective study we demonstrated the excellent operating characteristics of a non-bronchoscopic BAL technique using a nasogastric tube compared with the frequently cited fiberoptic bronchoscope-guided BAL.

There is currently no gold standard for the diagnosis of VAP and clinicians rely on clinical and bacteriologic strategies to manage patients with VAP4. The bacteriologic strategy uses quantitative cultures of lower respiratory secretions and has been associated with less use of antibiotics4. In a seminal paper, Fagon et al showed that an invasive strategy using FOB with quantitative cultures improves survival (14 days), and decreases antibiotic use7. Eventhough the bronchoscopically-guided BAL has several advantages, the most important being the ability to direct sampling into the desired lobe, it is important to emphasize its limitations in resource constrained settings. Fiberoptic bronchoscopes and qualified operators are not always readily available, thus potentially delaying pathogen-directed treatment with its harmful consequences17,18. Previous reports of «blind» invasive procedures have yield conflicting evidence, mostly because of variable methodologies, different thresholds of the quantitative studies, and reference standards11,19–26. Minutoli et al reported in the late 1980s the use of a nasogastric tube to do bronchoalveolar lavages in patients with the acquired immunodeficiency syndrome9. We extended their experience using this technique to obtain distal airway sample for bacterial cultures of patients with high clinical suspicion of VAP.

In an attempt to standardize a technique that should be simple, widely available, inexpensive, and with low risk of complications, we analyzed the performance of the nasogastric tube with quantitative cultures side-by-side with the bronchoscopy-directed BAL in the same patient with excellent results. By using the exact same lavage volume, quantitative threshold, and discarding the first aspirate, we obtained an excellent correlation of results between the two techniques. Furthermore, by using the same patient, and randomizing which technique to use first, as well as blinding the laboratory technician reading the cultures, we minimized bias. Our study also suggested that VAP is a diffuse disease involving multiple lobes, and samples obtained blindly have a comparable performance to FOB-guided samples11,20,21,27–30. Furthermore, histology-based reports suggest VAP is predominantly a dependent lung segment disease24 where is more likely that a nasogastric tube will go. Because the nasogastric tube has roughly the same size as a fiberoptic bronchoscope and unable to reach peripheral sections of the lung, we avoided complications such as pneumothorax.

Our study has several limitations, the two most important being that like any single center study, its results may not be generalizable to other settings. The second limitation of the study is the small sample size did not allow for subgroup analysis for specific admission diagnosis. However, the main objective of the study was to compare the microbiologic findings of the two techniques and we achieved that objective.

The results of our study have important implications in the care of patients with VAP in resourceconstrained settings, where the availability of bronchoscopes to confirm the diagnosis of VAP is limited.

We believe this innovative and simple technique should be validated in larger clinical trials, where antibiotic use, organ dysfunction improvement, and ultimately survival should be used as outcome measures.

Declaration of conflict of interest

All the authors reported no conflict of interest.

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Copyright © 2008. Sociedad Española de Medicina Intensiva, Critica y Unidades Coronarias (SEMICYUC) and Elsevier España, S.L.
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